What are Exosomes?

Exosomes are small structures secreted by most cell types in our bodies that facilitate important functions. They have the ability to transfer DNA, RNA, and proteins to other cells, thereby altering the function of the targeted cells. Exosomes are secreted by most cell types and contribute to functions including tissue repair, neural communication.

The history of Exosomes:

The first documentation of the word ‘exosome’ was in 1987 but researchers discovered these small vesicles being secreted from immature red blood cells called reticulocytes in 1983. They were thought of as little more than mobile trash cans whose job was to discard unwanted cellular components. There wasn’t much research done on exosomes until recently where researchers have started to recognize the medical potential of these cellular vehicles.

Just a trash can or something more?

After an almost 20 year gap of little research done on exosomes, studies started coming out that produced surprising findings. Subsequent research found that exosomes play an active part in cellular communication and instead of just disposing of waste they can carry proteins, RNA, growth factors and functional immune agents. Because of their physical structure and their ability to shuttle multiple products they can target cells that are near or far via blood channels.

Research being done today?

The explosion of exosome research has shown the great potential in medical applications. Regenerative medicine is a key area for the use of exosomes because of the growth factors they can shuttle. Growth factors are proteins that stimulate the growth of tissue and exosomes can deliver hundreds of these growth factors across a long distance. There is also promising areas of study in cancer research regarding exosomes and the ability to deliver antitumor responses which can initiate apoptosis (death of a cell) of these cancer cells. There is still more research to be done on exosomes but they are a bright new area of study in stem cell research, cellular health, and regenerative medicine.

ERECTUSXOME (TM) is used in our Patent pending procedure for the ERECTUS Shot without the need to use adipose-derived Cell Therapy and no need to do liposuction. Erectusxome are rich in natural growth factors and contains no stem cells

SEXOXOMES are used for our V Shot procedure for enhancement of female organ function. Sexoxomes are rich in natural growth factors and contains no stem cells

FOR MORE INFORMATION call 407 771 0404.

  • Johnstone, R. M., Adam, M., Hammond, J. R., Orr, L., & Turbide, C. (1987). Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). Journal of Biological Chemistry262(19), 9412-9420.
  • Johnstone, R. M. (2006). Exosomes biological significance: a concise review. Blood Cells, Molecules, and Diseases36(2), 315-321.
  • Simons, M., & Raposo, G. (2009). Exosomes–vesicular carriers for intercellular communication. Current opinion in cell biology21(4), 575-581.
  • Théry, C., Ostrowski, M., & Segura, E. (2009). Membrane vesicles as conveyors of immune responses. Nature Reviews Immunology9(8), 581-593.
  • Théry, C., Zitvogel, L., & Amigorena, S. (2002). Exosomes: composition, biogenesis and function. Nature Reviews Immunology2(8), 569-579.
  • Zitvogel, L., Regnault, A., Lozier, A., Wolfers, J., Flament, C., Tenza, D., … & Amigorena, S. (1998). Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell derived exosomes. Nature medicine4(5), 594-600.
  • Wolfers, J., Lozier, A., Raposo, G., Regnault, A., Théry, C., Masurier, C., … & Angevin, E. (2001). Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming. Nature medicine7(3), 297-303.
  • Taylor, D. D., & Gercel-Taylor, C. (2008). MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecologic oncology110(1), 13-21.
  • Tomasoni, S., Longaretti, L., Rota, C., Morigi, M., Conti, S., Gotti, E., … & Benigni, A. (2012). Transfer of growth factor receptor mRNA via exosomes unravels the regenerative effect of mesenchymal stem cells. Stem cells and development22(5), 772-780.
  • Skog, J., Würdinger, T., van Rijn, S., Meijer, D. H., Gainche, L., Curry, W. T., … & Breakefield, X. O. (2008). Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nature cell biology10(12), 1470-1476.
  • Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007). Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nature cell biology9(6), 654-659.
  • Cocucci, E., Racchetti, G., & Meldolesi, J. (2009). Shedding microvesicles: artefacts no more. Trends in cell biology19(2), 43-51.
  • Kosaka, N., Iguchi, H., Yoshioka, Y., Takeshita, F., Matsuki, Y., & Ochiya, T. (2010). Secretory mechanisms and intercellular transfer of microRNAs in living cells. Journal of Biological Chemistry285(23), 17442-17452.
  • Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006). Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Current protocols in cell biology, 3-22.
  • Raposo, G., & Stoorvogel, W. (2013). Extracellular vesicles: exosomes, microvesicles, and friends. The Journal of cell biology200(4), 373-383.
  • Pegtel, D. M., Cosmopoulos, K., Thorley-Lawson, D. A., van Eijndhoven, M. A., Hopmans, E. S., Lindenberg, J. L., … & Middeldorp, J. M. (2010). Functional delivery of viral miRNAs via exosomes. Proceedings of the National Academy of Sciences107(14), 6328-6333.
  • Stoorvogel, W., Kleijmeer, M. J., Geuze, H. J., & Raposo, G. (2002). The biogenesis and functions of exosomes. Traffic3(5), 321-330.
  • Miller, I. V., & Grunewald, T. G. (2015). Tumourderived exosomes: Tiny envelopes for big stories. Biology of the Cell107(9), 287-305.
  • Duijvesz, D., Luider, T., Bangma, C. H., & Jenster, G. (2011). Exosomes as biomarker treasure chests for prostate cancer. European urology59(5), 823-831.
  • Khan, N. A. (2014). Molecular? debris?: Trash or Treasure?. Journal of Nanomedicine & Biotherapeutic Discovery2014.
  • de Gassart, A., Geminard, C., Hoekstra, D., & Vidal, M. (2004). Exosome secretion: the art of reutilizing nonrecycled proteins?. Traffic5(11), 896-903.
  • Wendler, F., Bota-Rabassedas, N., & Franch-Marro, X. (2013). Cancer becomes wasteful: emerging roles of exosomes in cell-fate determination. Journal of extracellular vesicles2.
  • Théry, C., Boussac, M., Véron, P., Ricciardi-Castagnoli, P., Raposo, G., Garin, J., & Amigorena, S. (2001). Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. The Journal of Immunology166(12), 7309-7318.
  • Abusamra, A. J., Zhong, Z., Zheng, X., Li, M., Ichim, T. E., Chin, J. L., & Min, W. P. (2005). Tumor exosomes expressing Fas ligand mediate CD8+ T-cell apoptosis. Blood Cells, Molecules, and Diseases35(2), 169-173.
  • Bhatnagar, S., Shinagawa, K., Castellino, F. J., & Schorey, J. S. (2007). Exosomes released from macrophages infected with intracellular pathogens stimulate a proinflammatory response in vitro and in vivo. Blood110(9), 3234-3244.
  • Aline, F., Bout, D., Amigorena, S., Roingeard, P., & Dimier-Poisson, I. (2004). Toxoplasma gondii antigen-pulsed-dendritic cell-derived exosomes induce a protective immune response against T. gondii infection. Infection and immunity72(7), 4127-4137.
  • Chaput, N., & Théry, C. (2011 September).Exosomes: immune properties and potential clinical implementations. In Seminars in immunopathology (Vol. 33, No. 5, pp. 419-440). Springer-Verlag.
  • Bobrie, A., Colombo, M., Raposo, G., & Théry, C. (2011). Exosome secretion: molecular mechanisms and roles in immune responses. Traffic12(12), 1659-1668.
  • Skokos, D., Botros, H. G., Demeure, C., Morin, J., Peronet, R., Birkenmeier, G., … & Mécheri, S. (2003). Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo. The Journal of Immunology170(6), 3037-3045.
  • Tsao, C.-R., Liao, M.-F., Wang, M., Cheng, C.-M., & Chen, C.-H. (2014). Mesenchymal Stem Cell Derived Exosomes: A New Hope for the Treatment of Cardiovascular Disease? . Acta Cardiologica Sinica30(5), 395–400.
  • Batrakova, E. V., & Kim, M. S. (2015). Using exosomes, naturally-equipped nanocarriers, for drug delivery. Journal of Controlled Release : Official Journal of the Controlled Release Society219, 396–405.
  • Fatima, F., & Nawaz, M. (2015). Stem cell-derived exosomes: roles in stromal remodeling, tumor progression, and cancer immunotherapy. Chinese Journal of Cancer34, 46.
  • Natasha, G., Gundogen, B., Tan, A., Farhatnia, Y., Wu, W., Rajadas, J., Seifalian, A.  (2014). Exosomes as Immunotheranaostic Nanoparticles. Clinical Therapeutics. 36(6), 820-827.
  • Rustom A, R Saffrich, I Markovic, P Walther and HH Gerdes. (2004). Nanotubular highways for intercellular organelle transport. Science 303:1007–1010.
  • Théry, Clotilde. (2011). Exosome Explosion. The Scientist. Vol 25, Issue 7. 
  • Camussi G, MC Deregibus, S Bruno, V Cantaluppi and L Biancone. (2010). Exosomes/microvesicles as a mechanism of cell-to-cell communication. Kidney Int 78:838–848
  • Fevrier B and G Raposo. (2004). Exosomes: endosomal-derived vesicles shipping extracellular messages. Curr Opin Cell Biol 16:415–421
  • Image. Kimera Labs. www.amnio2x.com

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